Cultural, Molecular and Genetic Variability of Ustilaginoidea virens Causing Rice False Smut Disease

Rice false smut disease is caused by Ustilaginoidea virens, an ascomycetous fungi. Thirty isolates of U. virens showed well defined colonies on potato sucrose medium (PSA). The maximum colony diameter was found to be 85.68 mm with a growth rate 2.85 in the UV23 isolate and minimum 10.14 0.33 mm, which observed UV15 isolate. However, thirty exhibit various cultural morphological characteristics like color, pattern, elevation, chlamydospore formation. In cluster analysis, two major groups (I) (II) were formed. first group contains second 29UV isolates. Furthermore, division II includes subgroup IIa, 22 isolates, IIb, 7 PCR amplification done species-specific primer yielded products 380 bp 230 bp, respectively. results phylogenetic tree analysis revealed that I contained 5 28 isoates from China Japan. MAT1-1-1 product 250 18 Whereas MAT1-2-1 220 12 nucleotide divergence polymorphic sites (s), 10 mutations (Eta), 0.76515 diversity (k) -2.13885 for tajimas test value. Likewise, haplotype haplotype(h)groups, (hd) 0.4773. With 24 hap-1 most common. Similarly, clear variation between Indian Chinese as Japanese ones. genetic similarity coefficient matrix (0.9). whereas UV32 0.6, UV31 exhibited 0.5, UVA33 had 0.4,.These indicate variability UV China.